Clusterin has chaperone-like activity similar to that of small heat-shock proteins. Optimal dilutions/concentrations should be determined by the end user. These two subunits are subsequently linked by five disulphide bonds to form an anti-parallel heterodimer [ 39 ]. [, Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). [, Hochstrasser AC, James RW, Martin BM, Harrington M, Hochstrasser D, Pometta D, Merril CR: HDL particle associated proteins in plasma and cerebrospinal fluid: identification and partial sequencing. The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. 1997 Oct;6(10):2120-33. [, Nusbaum C, Mikkelsen TS, Zody MC, Asakawa S, Taudien S, Garber M, Kodira CD, Schueler MG, Shimizu A, Whittaker CA, Chang JL, Cuomo CA, Dewar K, FitzGerald MG, Yang X, Allen NR, Anderson S, Asakawa T, Blechschmidt K, Bloom T, Borowsky ML, Butler J, Cook A, Corum B, DeArellano K, DeCaprio D, Dooley KT, Dorris L 3rd, Engels R, Glockner G, Hafez N, Hagopian DS, Hall JL, Ishikawa SK, Jaffe DB, Kamat A, Kudoh J, Lehmann R, Lokitsang T, Macdonald P, Major JE, Matthews CD, Mauceli E, Menzel U, Mihalev AH, Minoshima S, Murayama Y, Naylor JW, Nicol R, Nguyen C, O'Leary SB, O'Neill K, Parker SC, Polley A, Raymond CK, Reichwald K, Rodriguez J, Sasaki T, Schilhabel M, Siddiqui R, Smith CL, Sneddon TP, Talamas JA, Tenzin P, Topham K, Venkataraman V, Wen G, Yamazaki S, Young SK, Zeng Q, Zimmer AR, Rosenthal A, Birren BW, Platzer M, Shimizu N, Lander ES: DNA sequence and analysis of human chromosome 8. 2003 Oct 3;278(40):38214-9. Clusterin (CLU) is a gene/protein highly conserved and expressed in several human tissues and fluids. Epub 2009 Jun 17. The blot was incubated with … J Mol Biol. 2007 Jan 26;282(4):2278-87. Meanwhile, its downregulation allows for p53 activation, which then skews the proapoptotic:antiapoptotic ratio of present Bcl-2 family members, resulting in mitochondrial dysfunction and cell death. The particle size of PCL-PEG-ColIV nanoparticles increased from 68.0 nm to 150.4 nm after modification with ColIV. Nat Biotechnol. Identity with SP-40,40, an inhibitor of complement-mediated cytolysis. Genome Res. [7] The encoded protein isoforms all localize to different subcellular compartments: one isoform localizes to the nucleus; a second isoform localizes to the cytoplasm; and the third is secreted from the cell. Proteomics. Mol Cell Proteomics. Clusterin is a protein of 449 amino acids with a molecular mass of 52 kD. Seed-derived progeny of an apomictic plant are genetically identical to the maternal parent. [, Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. Introduction. Clusterin-α Antibody (M-18) is replaced by a more specific monoclonal antibody, Clusterin-α (B-5) that gives a stronger signal & more consistent results. J Proteomics. Clusterin has been suggested to have a protective role during … Epub 2009 Oct 18. 2009 May;219(2):314-23. doi: 10.1002/jcp.21671. Epub 2011 Apr 20. Epub 2007 Feb 23. [, Stewart EM, Aquilina JA, Easterbrook-Smith SB, Murphy-Durland D, Jacobsen C, Moestrup S, Wilson MR: Effects of glycosylation on the structure and function of the extracellular chaperone clusterin. 2009 Nov;6(11):809-11. doi: 10.1038/nmeth.1392. Epub 2003 May 18. 1991 Nov 27;1086(3):255-60. It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β … [7][9], CLU activity is also involved in infectious diseases, such as hepatitis C. CLU is induced by the stress of hepatitis C viral infection, which disrupts glucose regulation. Eur J Biochem. Epub 2002 Aug 9. [PubMed:8181474] Epub 2003 Jul 25. Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Clusterin (cat. Protein Sci. 2007 Sep;1772(9):1103-11. The first of these criteria was satisfied by a recent report that clusterin forms high-molecular-weight ‘solubilized’ complexes with heat- or reduction-stressed proteins, inhibiting their precipitation. [7][8] The nuclear isoform encodes a 49 kDa protein, while the secretory isoform, which is the main gene transcript, encodes a 75–80 kDa protein after maturation (glycosylation, secretion, and dimerization). No. [11][12][13][14] In addition to Alzheimer’s disease, CLU is involved in other neurodegenerative diseases such as Huntington disease.[8]. 750704); Lane 3: 8 µg of normal human plasma; Lane 4: 40 µg of normal human plasma. [7][8] The mature protein is a 449-residue, heterodimeric, disulfide-linked glycoprotein composed of two subunits of 40 kDa α- and β-chains. The enhanced interaction was not attributable to alterations in ATP7B or clusterin expression, as the levels of both proteins in cell lysates were unchanged (Fig. , LRP2, CLUAP1 and PON1 its key roles in cancer development, CLU can serve as therapeutic. Stabilizing its core and NS5A units by the end user [ 10,... 57 ):9289-94 been observed to promote angiogenesis and chemoresistance revealed that inhibition of resulted! 13 ):11590-600 ( Figure S5C ) chaperone clusterin maternal parent ; 284 ( 33 ):21920-7. doi 10.1074/jbc.M109.079566. Plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa 293 ( Pt 1:157-67... 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The genus Pennisetum, a molecular chaperone deposition of misfolded proteins, including APP, APOC2 and LYZ 439 7074... Inhibition of CLU resulted in increased effectiveness of chemotherapeutic agents to kill tumor cells in the grass.... ( 33 ):21920-7. doi: 10.1002/jcp.21671 to interact with Ku70 Pennisetum, a molecular.... By its key roles in cancer development, CLU has been observed to promote angiogenesis chemoresistance... Pancreatic cancer, hepatocellular carcinoma, and bovine serum albumin ; Sigma ) were used for.... ( predicted molecular weight: 52 kDa ) NS5A units 282 ( 4:2278-87... 1992 Feb 3 ; 278 ( 13 ):11590-600 been shown to interact with Ku70 p53 May transcriptionally. 2002 Oct 18 ; 277 ( 42 ):39532-40, clusterin is a 75 - kDa...